AdapterRemoval¶
AdapterRemoval searches for and removes remnant adapter sequences from High-Throughput Sequencing (HTS) data and (optionally) trims low quality bases from the 3' end of reads following adapter removal. AdapterRemoval can analyze both single end and paired end data, and can be used to merge overlapping paired-ended reads into (longer) consensus sequences. Additionally, Additionally, AdapterRemoval can construct a consensus adapter sequence for paired-ended reads, if which this information is not available.
If you use AdapterRemoval v2, then please cite the paper:
Schubert, Lindgreen, and Orlando (2016). AdapterRemoval v2: rapid adapter trimming, identification, and read merging. BMC Research Notes, 12;9(1):88 http://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-016-1900-2
AdapterRemoval was originally published in Lindgreen 2012:
Lindgreen (2012): AdapterRemoval: Easy Cleaning of Next Generation Sequencing Reads, BMC Research Notes, 5:337 http://www.biomedcentral.com/1756-0500/5/337/
Contents:
- Installation
- Getting started
- Example usage
- Trimming single-end reads
- Trimming paired-end reads
- Multiple input FASTQ files
- Interleaved FASTQ reads
- Combining FASTQ output
- Different quality score encodings
- Trimming paired-end reads with multiple adapter pairs
- Identifying adapter sequences from paired-ended reads
- Demultiplexing and adapter-trimming
- Demultiplexing mode
- AdapterRemoval manpage
- Miscellaneous